Lymphocytic Choriomeningitis Virus (LCMV) Infection

Back

Background

Lymphocytic choriomeningitis virus (LCMV) was first isolated in 1933 from a patient suspected to have St. Louis Encephalitis.[1] It is a single-stranded RNA virus that belongs to the family Arenaviridae (so named because of their appearance on electron microscopy, which, owing to host ribosomes, resemble grains of sand).[2]

Other members of the Arenaviridae family include the Lassa virus (LASV) and the New World complex viruses (Junin, Machupo, Guanarito, Sabia). Infection with LCMV results in a febrile, self-limited, biphasic disease that is often complicated by aseptic meningitis. Infected but asymptomatic (carrier state) rodents, most commonly mice (Mus domesticus, Mus musculus), hamsters, and Guinea pigs, serve as reservoirs for LCMV.[3, 4]

LCMV is most commonly transmitted via inhalation of infected excreta. Direct contact and animal bites are a potential route of LCMV infection in pet handlers and laboratory technicians.[4, 5]

Pathophysiology

The initial viremia of LCMV infection (phase 1) extensively seeds extra-CNS tissue. The secondary viremia (phase 2) infects the meninges and, less commonly, the cortical tissue. The leptomeninges are infiltrated mainly by lymphocytes and histiocytes, with few neutrophils. In LCMV encephalitis, the same type of inflammatory cells is observed in the perivascular Virchow-Robin spaces. LCMV is not cytotoxic. It appears that the host's immune response to the infected cells produces the various manifestations of this disease. Natural killer (NK) cells are first to respond, followed by the production of interferon by cytotoxic T cells. LCMV antibodies become detectable during the second febrile episode. In addition, LCMV can suppress the production of acetylcholine neuronal cells in cell culture.[6, 7, 8, 9, 10]

LCMV may affect the autonomic nervous system, various sensory modalities, and cranial nerves. Rarely, the virus can cause long-term neurologic sequelae, including chronic headache, hydrocephalus, deafness, transverse myelitis, and Guillain-Barré syndrome.[11] Other organs, especially the testes, heart, and joints, may be involved. Orchitis is usually unilateral and develops 1-3 weeks after illness onset. Cardiac involvement can occur in the form of viral myocarditis or pericarditis. The metacarpophalangeal joint and the proximal interphalangeal joint are the most common sites of arthritis caused by LCMV. The objective swelling, redness, and pain resolve within a few weeks.[8, 9, 12]

Vertical transmission of LCMV during pregnancy has been associated with increased risk of spontaneous abortion. It can also cause a syndrome of hydrocephalus, chorioretinitis, and perivascular calcifications similar to that seen in congenital cytomegalovirus (CMV) infection and toxoplasmosis, potentially leading to mental retardation, microcephaly, seizures, and blindness.[13]

In solid organ transplant recipients with donor-derived infection (DDI), LCMV has been shown to cause severe illness characterized by multisystem organ failure.[14] Meningitis is a less-prominent feature in these individuals. Their high degree of morbidity and mortality can be attributed to profoundly decreased cell-mediated immunity due to immunosuppression.

Epidemiology

Frequency

United States

The exact incidence of LCMV infection is unknown, although the seroprevalence is approximately 5%. Local variations in seropositivity for LCMV (2%-5%) depend on the local rodent populations.[15] The true prevalence of LCMV infection is suspected to be higher because of underreporting and missed diagnoses; 10% or more cases of aseptic meningitis may be due to LCMV.[16] LCMV infection in humans is most common in autumn owing to migration of mice into warm structures.[9]

International

LCMV infections have been reported in North America, South America, Europe, Australia, and Japan.[9, 17, 18]

Mortality/Morbidity

LCMV infection carries a mortality rate of less than 1%. Death may be attributable to complications of encephalitis or to a hemorrhagic syndrome. As with other arenaviruses, immunosuppression may predispose to a syndrome of multisystem organ failure including hemorrhage.[16]

Race

LCMV infection has no racial predilection.

Sex

LCMV infection has no sexual predilection.

Age

LCMV infection is more common in young adults, although illness may occur in any age group.[9]

History

Clinical manifestations of lymphocytic choriomeningitis virus (LCMV) infection in immunocompetent individuals range from a flulike illness to severe CNS involvement with meningoencephalitis. Phase 1 of LCM typically manifests as fever and headache, often with lymphadenopathy and a maculopapular rash, resolving after 3-5 days. In many patients, a more severe headache returns within 2-4 days, associated with typical signs of aseptic meningitis.[6, 8, 9, 16]

Patients with LCMV infection may report a history of exposure to rodents, hamsters, or the excreta of these animals 1-3 weeks before the onset of symptoms. Infection is most common in the autumn. Smoking is a risk factor.[19] Approximately one third of LCMV infections cause no symptoms, and up to one half of infected individuals have a nonspecific febrile illness without neurologic involvement. The remainder of patients experience classic biphasic symptoms associated with LCMV infection and meningitis or encephalitis.

Initial nonspecific symptoms of LCMV infection include the following:

Symptoms may subside for 2-4 days and then recur with the following:

Immunosuppressed individuals (eg, solid organ transplant recipients) may develop a syndrome of multisystem organ involvement including the following:[20, 16]

Neurologic sequelae are rare but may include chronic headache, hydrocephalus, deafness, transverse myelitis, and Guillain-Barré syndrome.[11]

Complete recovery within 1-3 weeks is the rule, although convalescence may be prolonged.

Physical

Typical clinical features of LCMV infection are as follows:[6, 8, 9]

Atypical clinical features of LCMV infection include the following:

Causes

Infection is caused by the lymphocytic choriomeningitis virus (LCMV), a member of the family Arenaviridae.

Transmission is generally via inhalation of LCMV virions in the aerosolized excreta (urine or feces) from chronically infected rodents.[9]

Transmission is also possible through close contact with infected animals, via direct inoculation through the skin or mucous membranes.

Populations at high risk of LCMV infection include the following:

Laboratory Studies

Lymphocytic choriomeningitis virus (LCMV) infection is initially diagnosed based on a suggestive history that is confirmed by various laboratory investigations.[9]

Complete blood cell (CBC) count may reveal leukopenia and thrombocytopenia early in the course of illness.

The preferred diagnostic modality is assessment of acute and convalescent immunoglobulin M (IgM) and immunoglobulin G (IgG) titers from both the serum and cerebrospinal fluid (CSF). The sensitivity of enzyme-linked immunosorbent assay (ELISA) is greater than that of immunofluorescence (IFA)–based assays. Complement fixation is insensitive and should not be used.[15]

Immunohistochemical staining, virus culture, and reverse transcription-polymerase chain reaction (RT-PCR) of tissues may be useful.

Typical findings of lumbar puncture are as follows:[9]

Medical Care

No antiviral agents have undergone clinical trials for the treatment of lymphocytic choriomeningitis virus (LCMV) infection.

Early diagnosis and supportive care (eg, fluid replacement, NSAID therapy) are essential, particularly in immunocompromised patients. Reduce immunosuppression, when feasible.

No specific drug treatment is indicated in most cases of LCMV infection. Most patients improve spontaneously within 1-3 weeks with no sequelae.

Ribavirin has in vitro activity against LCMV and has been used with success in transplant recipients with severe disease. Intravenous ribavirin is not commercially available. Oral ribavirin is dosed based on ideal body weight and renal function. Patients should be monitored carefully for potential toxicity, including hemolytic anemia, while receiving ribavirin.[20, 14]

Favipiravir (T-705), a selective inhibitor of RNA-dependent RNA polymerase (RdRp), has been shown to inhibit LCMV in vitro. It has also demonstrated promising efficacy at reducing mortality of other arenavirus infections in animal models. Further study is needed to ascertain if favipiravir could be safely used to treat infections with arenaviruses, including LCMV in humans.[21, 22]

Further Inpatient Care

Patients with severe meningoencephalitis are usually hospitalized.

Deterrence/Prevention

Rodent control measures decrease the frequency of lymphocytic choriomeningitis virus (LCMV) infection.[9]

Laboratory personnel who handle mice or hamsters are at increased risk for LCMV infection. No established method of preventing infection in these situations exists. Prudence dictates the use of gloves when handling these animals, especially if the person's hands are abraded. If the risk of infection is high, consider the use of a personal respirator.

No method is effective to prevent transmission by organ transplantation since determination of pet rodent ownership by the donor is neither sensitive or specific. Testing tissue with RT-PCR and immunohistochemical analysis is extremely expensive and may not necessarily be effective.

Prognosis

Lymphocytic choriomeningitis (LCM) is rarely fatal; the overall prognosis is excellent.

Patients with encephalitis are at higher risk for neurologic sequelae.

Convalescence may be prolonged, with continuing dizziness, somnolence, and fatigue.

Severe disease leading to death has been reported in immunocompromised patients and organ transplant recipients (7 of 8 infected patients died).[23]

Patient Education

Avoid exposure to rodent secreta and excreta.

Author

Philip J McDonald, MD, Fellow, Division of Infectious Diseases, Department of Internal Medicine, Detroit Medical Center, Wayne State University School of Medicine

Disclosure: Nothing to disclose.

Coauthor(s)

Pranatharthi Haran Chandrasekar, MBBS, MD, Professor, Chief of Infectious Disease, Department of Internal Medicine, Wayne State University School of Medicine

Disclosure: Nothing to disclose.

Specialty Editors

Francisco Talavera, PharmD, PhD, Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Received salary from Medscape for employment. for: Medscape.

John L Brusch, MD, FACP, Assistant Professor of Medicine, Harvard Medical School; Consulting Staff, Department of Medicine and Infectious Disease Service, Cambridge Health Alliance

Disclosure: Nothing to disclose.

Chief Editor

Burke A Cunha, MD, Professor of Medicine, State University of New York School of Medicine at Stony Brook; Chief, Infectious Disease Division, Winthrop-University Hospital

Disclosure: Nothing to disclose.

Additional Contributors

Mark R Wallace, MD, FACP, FIDSA, Infectious Disease Physician, Skagit Valley Hospital, Skagit Regional Health

Disclosure: Nothing to disclose.

Rupal M Mody, MD, MPH, Staff Physician

Disclosure: Nothing to disclose.

Acknowledgements

Diane H Johnson, MD Assistant Director, Assistant Professor, Department of Internal Medicine, Division of Infectious Diseases, Winthrop-University Hospital, State University of New York at Stony Brook School of Medicine

Diane H Johnson, MD is a member of the following medical societies: American College of Physicians, American Medical Association, American Medical Women's Association, American Society for Microbiology, and Infectious Diseases Society of America

Disclosure: Nothing to disclose.

References

  1. Armstrong C, Lillie RD. Experimental lymphocytic choriomeningitis of monkeys and mice produced by a virus encountered in studies of the 1933 St. Louis encephalitis epidemic. Public Health Res. 1933. 49:1019–1022.
  2. Murphy FA, Whitfield SG. Morphology and morphogenesis of arenaviruses. Bull World Health Organ. 1975. 52 (4-6):409-19. [View Abstract]
  3. Brown D, Lloyd G. Zoonotic virus. Infectious Diseases. Philadelphia, Pa: Mosby; 1999. 11.1-11.14.
  4. Childs JE, Glass GE, Korch GW, et al. Lymphocytic choriomeningitis virus infection and house mouse (Mus musculus) distribution in urban Baltimore. Am J Trop Med Hyg. 1992 Jul. 47(1):27-34. [View Abstract]
  5. Pedrosa PB, Cardoso TA. Viral infections in workers in hospital and research laboratory settings: a comparative review of infection modes and respective biosafety aspects. Int J Infect Dis. 2011 Jun. 15(6):e366-76. [View Abstract]
  6. Cunha BA. Meningitis. Schlossberg D, ed. Central Nervous System Infections. New York, NY: Springer-Verlag; 1990.
  7. Farmer TW, Janeway CA. Infection with the virus of lymphocytic choriomeningitis. Medicine (Baltimore). 1942. 2:11.
  8. McKee KT Jr. Hemorrhagic fever virus. Infectious Diseases. 2nd ed. Philadelphia, Pa: WB Saunders Co; 1998. 2249-65.
  9. Seregin A, Yun Nadezhda, Paessler S. Lymphocytic Choriomeningitis, Lassa Fever, and the South American Hemorrhagic Fevers (Arenaviruses). Bennett JE, Dolin R, Blaser MJ. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 8th ed. Philadelphia, PA: Elsevier Saunders; 2015. 2031-2037.
  10. Wilson MR, Peters CJ. Diseases of the central nervous system caused by lymphocytic choriomeningitis virus and other arenaviruses. Handb Clin Neurol. 2014. 123:671-81. [View Abstract]
  11. Souders HT, Byler D, Marupudi N, Patel R, McSherry G. Protracted symptoms in lymphocytic choriomeningitis: a case report. J Child Neurol. 2015 Apr. 30 (5):644-7. [View Abstract]
  12. Oldstone MB. Lessons learned and concepts formed from study of the pathogenesis of the two negative-strand viruses lymphocytic choriomeningitis and influenza. Proc Natl Acad Sci U S A. 2013 Mar 12. 110(11):4180-3. [View Abstract]
  13. Barton LL, Mets MB, Beauchamp CL. Lymphocytic choriomeningitis virus: emerging fetal teratogen. Am J Obstet Gynecol. 2002 Dec. 187 (6):1715-6. [View Abstract]
  14. Mathur G, Yadav K, Ford B, Schafer IJ, Basavaraju SV, Knust B, et al. High clinical suspicion of donor-derived disease leads to timely recognition and early intervention to treat solid organ transplant-transmitted lymphocytic choriomeningitis virus. Transpl Infect Dis. 2017 Aug. 19 (4):[View Abstract]
  15. Lapošová K, Lukáčiková Ľ, Ovečková I, Pastoreková S, Rosocha J, Kuba D, et al. Development and application of ELISA for the detection of IgG antibodies to lymphocytic choriomeningitis virus. Acta Virol. 2016 Jun. 60 (2):143-50. [View Abstract]
  16. Peters CJ. Lymphocytic choriomeningitis virus--an old enemy up to new tricks. N Engl J Med. 2006 May 25. 354(21):2208-11. [View Abstract]
  17. Smith AL, Singleton GR, Hansen GM, Shellam G. A serologic survey for viruses and Mycoplasma pulmonis among wild house mice (Mus domesticus) in southeastern Australia. J Wildl Dis. 1993 Apr. 29 (2):219-29. [View Abstract]
  18. Takagi T, Ohsawa M, Yamanaka H, Matsuda N, Sato H, Ohsawa K. Difference of two new LCMV strains in lethality and viral genome load in tissues. Exp Anim. 2017 Aug 5. 66 (3):199-208. [View Abstract]
  19. Knust B, Ströher U, Edison L, Albariño CG, Lovejoy J, Armeanu E, et al. Lymphocytic choriomeningitis virus in employees and mice at multipremises feeder-rodent operation, United States, 2012. Emerg Infect Dis. 2014 Feb. 20 (2):240-7. [View Abstract]
  20. Fischer SA, Graham MB, Kuehnert MJ, Kotton CN, Srinivasan A, Marty FM. Transmission of lymphocytic choriomeningitis virus by organ transplantation. N Engl J Med. 2006 May 25. 354(21):2235-49. [View Abstract]
  21. Mendenhall M, Russell A, Juelich T, Messina EL, Smee DF, Freiberg AN, et al. T-705 (favipiravir) inhibition of arenavirus replication in cell culture. Antimicrob Agents Chemother. 2011 Feb. 55 (2):782-7. [View Abstract]
  22. Furuta Y, Komeno T, Nakamura T. Favipiravir (T-705), a broad spectrum inhibitor of viral RNA polymerase. Proc Jpn Acad Ser B Phys Biol Sci. 2017. 93 (7):449-463. [View Abstract]
  23. Razonable RR. Rare, unusual, and less common virus infections after organ transplantation. Curr Opin Organ Transplant. 2011 Dec. 16(6):580-7. [View Abstract]
  24. Barton LL. LCMV transmission by organ transplantation. N Engl J Med. 2006 Oct 19. 355(16):1737; author reply 1737-8. [View Abstract]
Diagnosis Season Usual Source Relative Bradycardia Pharyngitis Diarrhea Parotitis Orchitis CSF Glucose level
LCMV infectionFall/winterMouse, hamster++/--+/-+/-Normal or decreased
Typhoid fever Year-roundFood, water+++ (late)--Normal
Enteroviral illness SummerWater-++--Normal
Arboviral illnessSummerMosquito-----Normal
Leptospirosis Summer/fallDogs, rats-----Normal
Influenza WinterPerson-+---Normal
Mumps Winter/springPerson---++/-Normal or decreased