Sir James Paget first described Paget disease (PD) of the breast in 1874. He reported a chronic eczematous disease on the skin of the nipple and the areola in 15 women, with an associated intraductal carcinoma of the underlying mammary gland. In 1881, Thin illustrated the first histologic description of PD.
Mammary PD occurs almost exclusively in women; involvement of the male breast is rarely reported and may be more aggressive.[1, 2] Patients with PD frequently present with a chronic, eczematous rash on the nipple and adjacent areolar skin. Proper recognition of this disorder is required to initiate an appropriate workup (eg, skin biopsy) for differentiating it from other benign inflammatory dermatoses and for detecting an underlying breast carcinoma.
A similar disease involving the skin of female and male external genitalia (ie, vulva, glans penis) is known as extramammary Paget disease.[3] The histologic features of mammary PD and extramammary PD are similar; however, the histogenesis and the pathogenesis are different.
The pathogenesis of mammary PD and the origin of Paget cells were once controversial. It is now widely accepted that mammary PD is always associated with an underlying carcinoma of the breast. By thorough histologic examination, Muir documented intraepidermal extension of malignant ductal epithelial cells through the lactiferous ducts and ductules into the epidermis (epidermotropism).[4] The findings are the basis of the epidermotropic theory of mammary PD.
Malignant epithelial (Paget) cells infiltrate and proliferate in the epidermis, causing thickening of the nipple and the areolar skin. These tumorous epithelial cells are derived from luminal lactiferous ductal epithelium of the breast tissue (see the image below).
View Image | Schematic diagram of female breast depicting widely accepted concept of pathogenesis of mammary Paget disease. Malignant Paget cells are derived from .... |
The cells possess microscopic features of glandular cells. Paget cells and underlying ductal carcinoma cells have been shown to be positive for the oncogene HER2/neu, suggesting common genetic alterations for both the epidermal and breast tumor cells.
It has been speculated that Paget cells may derive from glandular stem cells or epidermal Toker cells (clear cells of the nipple epithelium).[5] Toker cells have been found in about 10% of normal nipples and rarely in supernumerary nipples on the mild line and apocrine bearing areas.[6] Like Paget cells of both mammary and extramammary sites, Toker cells contain prominent clear (vacuolated) cytoplasm, and they are considered benign counterparts of Paget cells.
Mammary Paget cells are malignant epithelial cells derived from underlying ductal adenocarcinoma of the breast that invade into the skin of nipple and areolar areas. Toker cells are benign and may sometimes proliferate, resulting in a condition known as clear cell papulosis.[6]
Kuan et al[7] reported on the immunohistochemical expression of apomucin MUC1, MUC2, and MUC5AC in PD and concluded that both epidermal Paget cells and underlying ductal carcinoma exhibit the same phenotypic apomucins that are also expressed by the Toker cells.
Morandi et al[8] reported that the chromosomal alterations seen in Paget cells (eg, loss of heterozygosity and mitochondrial DNA displacement loop sequence analysis) are different from those seen in underlying breast carcinoma cells. They suggested that the epidermal Paget cells are genetically different from those of breast carcinoma.
A concept of a “collision” between the neoplastic lesion of mammary PD and the underlying carcinoma is therefore presented. However, this concept is based on only a few cases. Further studies are necessary to arrive at a more definitive conclusion concerning collision lesions.
Paget cells often express cell markers that mimic those of the underlying breast carcinoma, including glandular epithelial cell markers (eg, low-molecular-weight cytokeratins or cellular adhesion molecule [CAM] 5.2).
They also express tumor markers, including carcinoembryonic antigen (CEA); Ca 15-3 (milk fat globule protein); oncogenes (TP53,c-erb B-2; about 85% of cases tested for c-erb B-2 are positive); and other cell markers, such as epithelial membrane antigen (EMA; all cases tested are EMA positive) and gross cystic disease fluid protein (GCDFP-15) found in tumor cells of ductal carcinoma of the breast.
Paget cells also share similar immunohistochemical characteristics with eccrine and apocrine sweat gland epithelium. Paget cells are periodic acid-Schiff (PAS) positive and diastase resistant; and they are Alcian blue positive at pH 2.5 in 32% and 18%, respectively.
Advances in immunohistochemistry further define the histogenesis of Paget cells. Cytokeratin 7 (CK7) has been proposed as a specific and nearly 100% sensitive marker for mammary PD; whereas cytokeratin 20 (CK20) is negative in mammary Paget cells, about 33% of cases of extramammary PD express this marker. However, there is a rare CK7-negative variant of Paget disease.[9] If in doubt, staining positive for GATA3 might be diagnostically beneficial.
The mechanism by which large neoplastic cells of glandular origin (ie, the Paget cells) infiltrate and spread to the overlying epidermal layers of the nipple and adjacent areola is induced by a mobility factor (heregulin-alpha) that acts through the HER2/neu receptor.[10]
Normal epidermal keratinocytes produce and release heregulin-alpha. This factor plays a significant role in the pathogenesis of PD. Paget cells express heregulin receptors HER2/neu and coreceptors HER3 and HER4. These receptor complexes on Paget cells bind heregulin-alpha, the mobility factor, resulting in the chemotaxis of breast ductal carcinoma cells. This process, in turn, causes migration and infiltration of Paget cells into the overlying epidermis of the nipple and the areolar skin.
Molecular studies have shown that abnormal plakoglobin (one of the cell-to-cell adhesion proteins) may be involved in the formation of some cases of PD of the breast and the vulva; and reduced expression of E-cadherin (another cell-cell adhesion protein) may have a role in the pathogenesis of extramammary PD.[11]
In the United States, about 1-4% of female breast carcinoma cases are associated with PD of the nipple, the areola, and the surrounding skin; nearly 100% of mammary PD cases are associated with an underlying carcinoma, either in situ (intraductal, 10%) or infiltrating cancer (90%). Occasional cases of PD have been reported in ectopic breast or supernumerary nipples.[12] Worldwide, t he exact frequency of mammary PD is unclear.
In female patients with mammary PD, ages range from 24 to 84 years, with a mean age at diagnosis of 55 years; the average age range is 53-59 years. The average patient age is 5-10 years older for patients with mammary PD than for individuals with breast carcinoma. The age of onset in male patients is generally in the fifth and sixth decades, with a range of 48-80 years.
Mammary PD occurs almost exclusively in females; PD of the male breast is extremely rare. The estimated occurrence of male breast carcinoma is only 1% of the rate in females. A rare case of PD in the male breast was recorded after treatment of a prostate carcinoma with estrogen. No racial predisposition is reported for mammary PD.
The prognosis for patients with mammary PD is related to disease stage and appears to be similar to that of women with other types of breast cancer.
Survival is related to the presence or the absence of a palpable breast tumor. When present, the prognosis is the poorest.[13] In one study, 31 (62%) of 50 patients with mammary PD presented with a detectable breast mass.
One half (50%) of patients with PD presenting with a palpable breast mass have associated axillary lymph node metastasis. Two thirds of patients with axillary node metastasis were reported to have a palpable breast mass, whereas one third of patients with axillary metastasis did not have a palpable mass.
Even in patients with mammary PD and no underlying tumor, 30% may develop an invasive carcinoma at a later date, and 20% of patients already have an associated in situ carcinoma of the breast. However, other reports indicate that no axillary metastases were detected in patients without a palpable breast mass.
Patients with an identifiable associated underlying breast tumor have a survival rate of 38-40% at 5 years and a survival rate of 22-33% at 10 years. The death rate for metastatic breast carcinoma in patients with mammary PD and underlying cancer is 61.3%, with a 10-year cumulative survival rate of 33%.
The reported survival rate of patients with PD without a palpable breast tumor (prior to surgery) ranges from 92% to 94% at 5 years and from 82% to 91% at 10 years.
Mastectomy is the standard treatment of mammary Paget disease. Conservative treatment with preservation of the nipple-areola complex results in a higher rate of recurrence than treatment by mastectomy.
A small number of patients with mammary PD who did not have a palpable breast mass and who had a negative mammogram underwent conservative excision of the involved nipple-areola with a wedge resection of the underlying breast tissue and remained disease free at 10-year follow-up.
Rare cases of recurrent mammary PD have been reported in patients who underwent partial nipple excision; however, no patient developed breast parenchymal recurrence at a mean follow-up of 36 months.
In a study of patients with mammary PD (without clinical or mammographic evidence of breast tumor) who were treated with radiation therapy alone or excision plus radiation, 3 of 20 patients developed recurrent disease in the nipple-areola at a mean follow-up of 7.5 years. Those patients eventually underwent a mastectomy.
According to Osteen,[14] 9 (11.4%) of 79 patients treated by local excision (with or without radiation therapy) developed recurrences.
Patients should be informed that PD of the nipple-areola complex is a rare form of breast cancer that is clinically characterized by eczematous changes in the nipple. Clinical symptoms include erythema, itching, a burning sensation, thickening of the skin, inversion of the nipple, and sometimes a bloody nipple discharge. Usually, symptoms are present for 6 months or more before the detection of an underlying breast cancer. A biopsy should promptly be performed on all suspicious lesions of the nipple area for accurate diagnosis and treatment.
Failure to educate patients about the potential for invasive breast carcinoma associated with mammary PD is a pitfall; without this knowledge, patients may delay further diagnostic mammography examination and possibly delay detection of an underlying breast cancer.
For patient education resources, see the Women’s Health Center and the Cancer and Tumors Center, as well as Breast Lumps and Pain, Breast Self-Exam, Breast Cancer, and Mastectomy.
Patients with mammary Paget disease (PD) present with a relatively long history of an eczematous skin lesion or persistent dermatitis in the nipple and adjacent areas.[15] Eczematous skin lesions are associated with several symptoms, including the following[16] :
Early symptoms and signs of mammary PD include the following:
Symptoms of pain, itching, and a burning sensation prompt patients to seek medical attention.
Scaly, erythematous, crusty, and thickened plaques on the nipple, spreading to the surrounding areolar areas, are typical (see the images below). Axillary PD can arise from underlying axillary accessory breast tissue.[17]
View Image | Biopsy-proven Paget disease involving nipple of 56-year-old woman. Patient noted erythematous, swollen, enlarged nipple with focal ulceration and oozi.... |
View Image | Mammary Paget disease (PD) affecting 48-year-old woman. Patient had experienced prolonged history of chronic eczematous dermatitis of nipple and areol.... |
An erythematous patch in mammary PD is usually sharply demarcated and infiltrated (unlike eczematous dermatitis). Retraction of the nipple or the presence of palpable nodules indicates an underlying breast cancer. Serosanguineous discharge from the nipple may be present. Lesion size ranges from 3 mm to 15 cm in diameter; the mean size is 2.8 cm in diameter. Nipple invagination is sometimes seen (see the image below).
View Image | Nipple invagination, deformed nipple-areola complex, marked erythema, and alternating hyperpigmentation and hypopigmentation noted in adjacent skin of.... |
Nipple changes are associated with an underlying carcinoma of the breast (ie, in situ, infiltrating ductal carcinoma) in more than 98% of patients; as many as two thirds of patients have a palpable breast tumor.
Unilateral involvement is the rule; however, bilateral mammary PD has occasionally been reported.[18] Rare cases of female patients with PD of supernumerary nipples have been reported.[19]
Pigmented mammary PD and pigmented extramammary PD are rare clinical entities in both males and females. These diseases may mimic malignant melanoma both clinically and histopathologically. They may also mimic melanoma on dermatoscopic examination.[20] In pigmented lesions of PD, increased numbers of benign melanocytes are present, which may interfere with the correct diagnosis of PD, resulting in misinterpretation of this condition as malignant melanoma.[21]
Sonographic abnormalities in the nipple-areolar complex may be evident.[27]
As this neoplasm may be multifocal and multicentric, MRI has been recommended to evaluate the true extent of disease.[28]
Radiographic changes seen in mammary Paget disease (PD) include the following:
About 50-70% of patients with biopsy-proven mammary PD show positive findings on mammography; image-guided biopsy is assisted by positive results on mammography.
Ninety-four percent of patients with biopsy-proven PD as the only physical finding had an underlying carcinoma, and nearly 60% had unifocal disease. However, negative preoperative mammography findings did not reliably exclude an underlying carcinoma. Statistical evidence suggests that in the setting of negative mammography findings, magnetic resonance imaging (MRI) of the involved breast can detect otherwise occult PD and thus facilitate treatment planning for patients with PD.[29]
Scrape cytology has been suggested as a noninvasive and reliable, rapid diagnostic screening method for mammary PD. Scrape the affected area of the nipple with a glass slide or a wooden spatula and stain the smears with Papanicolaou or Giemsa stain. The presence of large cells with a high nuclear-to-cytoplasmic ratio, occasional acinar formation, and intracytoplasmic vacuoles is diagnostic for malignant Paget cells.
Using histochemical staining methods for the presence of epithelial mucin and immunoperoxidase stains (eg, anti-carcinoembryonic antigen [CEA]) enhances cytologic results and confirms the diagnosis of mammary PD. Drying artifact on the microscopic slides may produce both false-positive and false-negative results.
Punch, wedge, or excisional biopsy of the lesional skin of the nipple-areola complex to include the dermal and subcutaneous tissue for detailed microscopic examination provides an adequate sample for the accurate diagnosis of mammary PD.
Several variants of PD of the breast show identical histopathologic features. The epidermis exhibits hyperkeratosis, parakeratosis, and acanthosis. Infiltration occurs by variable numbers of large, rounded or ovoid, signet-ring forms and sometimes mucin-positive, malignant-appearing tumor cells that are present in all layers of the epidermis. The tumor cells contain abundant pale-staining or sometimes eosinophilic cytoplasm and large vesicular-to-hyperchromatic nuclei with prominent nucleoli (see the images below).
View Image | Photomicrograph of mammary Paget disease lesion. Note nests of malignant Paget cells predominantly involving lower layers of epidermis. Cytoplasm of t.... |
View Image | Composite photomicrograph of mammary Paget disease depicting nests, islands, and individual tumor cells in epidermis (left; hematoxylin-eosin, ×250), .... |
The malignant cells infiltrating the epidermis of the nipple are CK7 positive, exhibiting a typical invasive “shotgun” pattern. Epidermal keratinocytes are negative for CK7.
Mitotic figures are occasionally identified. The cytoplasm may contain periodic acid-Schiff (PAS)–positive, diastase-resistant granules, indicating the presence of neutral mucopolysaccharides. Less often, acid mucopolysaccharides (sialomucin) may be identified by Alcian blue reaction at pH 2.5, but not at lower pH (0.4) or by aldehyde Fuchsin.
Melaninlike pigment that is dihydroxyphenylalanine (DOPA)-negative is occasionally noted. Paget cells are devoid of intercellular bridges on hematoxylin and eosin (H&E)–stained sections. They often compress the basal keratinocytes that lie between the Paget cells and the papillary dermis. Paget cells are arranged singly or in a nested pattern, with occasional ductal formations.
Masses of large cells with numerous mitoses are observed in the advancing border of mammary PD. In the ulcerated lesions of mammary PD, the epidermis is totally replaced by Paget cells. Paget cells do not invade the dermis directly; however, they frequently extend along the follicular and sweat gland epithelia.
A large biopsy or excision may demonstrate the presence of epidermal Paget cells and an underlying infiltrating or intraductal carcinoma of the breast. The epidermal involvement by Paget cells is not always immediately adjacent to an underlying breast carcinoma.
The several histologic variants of PD are as follows:
Tumorous Paget cells are negative for estrogen and progesterone receptor sites, though one half of breast carcinomas are positive for these hormone receptors. In cases of positive estrogen and progesterone receptors in an underlying breast carcinoma, the overlying Paget disease is negative for these receptors. Paget cells are negative for mammary gland markers, such as lysozyme, k-casein, and alpha-lactalbumin.
The dermis contains a dense infiltrate of lymphocytes, histiocytes, plasma cells, and occasionally eosinophils.
The ultrastructural features of Paget cells are those of glandular epithelial cells. The pale cytoplasm of Paget cells lacks the dense CK filaments and keratohyalin granules of keratinocytes. Numerous free ribosomes, lysosomes, enlarged mitochondria, prominent smooth and rough endoplasmic reticulum, tonofilaments, Golgi membranes, and microvilli are present on the cell membrane (see the image below).
View Image | Low-power view of transmission electron micrograph displaying malignant Paget cells in lower layer of epidermis. Note large Paget cell containing ovoi.... |
Desmosomal attachments between Paget cells and adjacent keratinocytes are fewer and smaller than those between keratinocytes. Paget cells do not directly contact the basal lamina, and no gap junctions or tight junctions are present.
The cytoplasm of Paget cells contains numerous rounded, membrane-bound mucin granules of varying electron density (see the image below).
View Image | Cytoplasm of malignant Paget cell is packed with numerous rounded, membrane-bound mucin granules with various electron densities (uranyl acetate and l.... |
The intraductal carcinoma underlying mammary PD typically shows distended lactiferous ductal lumen by pleomorphic tumor cells with hyperchromatic nuclei, a high nuclear-to-cytoplasmic ratio, and a frequent gland-in-gland (cribriform) pattern (see the image below).
View Image | Photomicrograph of intraductal carcinoma of breast underneath Paget disease of nipple in 56-year-old woman. Note expansion of ductal lumen, which is f.... |
Both allergic contact dermatitis and irritant contact dermatitis and fixed drug eruption of the nipple-areola complex show histologic features of an inflammatory dermatosis. No Paget cells are seen in these conditions.
Nipple duct adenoma or erosive adenomatosis of the nipple may mimic mammary PD clinically; however, the histologic features of the former are those of a benign neoplasm of the major nipple ducts. The adenoma cells are negative for c-erb B-2, which is positive in 85% of cases of PD tested.
The following 2 histologic features differentiate Paget cells from melanoma cells:
View Image | Photomicrograph of malignant melanoma in situ of skin displays prominent intraepidermal pagetoid spread. Note that melanoma cells are present in all l.... |
Immunohistochemistry allows definitive diagnosis of mammary PD. Paget cells can be demonstrated by immunohistochemical methods using several antibodies to cell surface and cytoplasmic markers (eg, low-molecular-weight keratins found in simple epithelia, EMA, c-erb B-2, polyclonal pCEA+).[32]
Paget cells differ from melanoma cells by negative cytoplasmic epithelial phenotype markers (including pancytokeratin), positive homatropine methylbromide (HMB-45), and positive Melan-A (Mart-1) activity in the melanoma cells.[11] Paget cells show negative staining by anti-S-100 protein, which serves as a differentiating feature from malignant melanoma in situ. Most Paget cases are positive for cytokeratin 7 (CK7) and gross cystic disease fluid protein (GCDFP-15).
Toker (clear) cell hyperplasia of the nipple and the areola is a benign condition without well-defined clinical manifestations. Unlike the Paget cells, the large clear cells do not contain epithelial mucin, and no association with an underlying breast carcinoma exists. Benign Toker cells are positive for CK7[33] but negative for CEA and S-100 protein.
The similarity of the immunophenotypes suggests that Toker cells may be the origin of intraepithelial Paget cells.[6] In cases of florid papillomatosis of the nipple, some CK7-positive cells may be found in the epidermis, a pitfall to be aware of when diagnosing PD of the nipple. Furthermore, the intraepidermal portion of the nipple ducts can be a pitfall for intraepidermal CK7-positive cells.
Pagetoid squamous cell carcinoma in situ (Bowen disease) of the breast is rare, and it can be distinguished from Paget disease by negative CK7, positive K903, and positive p16 activity in the former and the reverse result for these antibodies in the latter. CEA is positive in Paget cells and negative in keratinocytes, thus differentiating Paget disease from Bowen disease.
Pseudo-Paget disease may, on occasion, be seen in the major nipple ducts. Large histiocytes infiltrate the epithelium and impart a histologic pattern mimicking Paget disease. The large cells are CK7 negative and strongly positive for CD68.
Mammary Paget disease has been classified into 4 clinical stages.
Of all patients with mammary PD, 40-50% have either stage 1 disease or stage 2 disease. These patients have no palpable breast tumor. A palpable breast tumor is a rule in stage 3; more than half of patients have coexisting axillary lymph node involvement. Patients with breast carcinoma previously treated by local excision and radiation therapy may present with PD of the nipple.
Mastectomy (radical or modified) and lymph node clearance are appropriate therapies for patients with mammary Paget disease (PD) with a palpable mass and underlying invasive breast carcinoma.[28, 34] As many as two thirds of patients are reported to have axillary lymph nodes positive for metastasis. Noninvasive breast carcinoma (in situ carcinoma) is found in about 65% of patients with mammary PD without a palpable mass.
Conservative management includes a combination of local excision of the nipple, wedge resection of the underlying breast, and radiation therapy. The number of patients treated by one or more conservative measures (eg, nipple excision and wedge excision of the underlying breast, cone excision, radiation therapy) is small. Breast conservation therapy with lumpectomy and radiation is an effective option, compared with mastectomy, for patients with Paget disease–ductal carcinoma in situ and Paget disease–invading ductal carcinoma.[35]
Patients who underwent cone excision and elective tamoxifen therapy had recurrences after an average follow-up of 4.6 years; some developed metastases. Therefore, cone excision is not sufficient therapy for patients with disease limited to the nipple.
Wide local excision with axillary node sampling is recommended for patients with or without a clinical mass.
Radiation therapy alone does not always control occult breast cancer; however, it may be used for patients who refuse mastectomy or those who are medically unfit for surgery.
The goals of pharmacotherapy are to reduce morbidity and prevent complications. A Cochrane review of aminolevulinic acid photodynamic therapy for mammary and extramammary Paget disease identified 21 retrospective and two prospective noncomparative studies with a total of 99 patients. The results suggest that this modality might be considered as adjunctive therapy for selected patients.[36]
Clinical Context: Aminolevulinic acid is a metabolic precursor of the photosensitizer protoporphyrin lX. When exposed to light of appropriate wavelength and energy, it induces local cytotoxicity through a photodynamic reaction.
These agents provide a photodynamic treatment for low-risk malignant cells and may improve morbidity.
Schematic diagram of female breast depicting widely accepted concept of pathogenesis of mammary Paget disease. Malignant Paget cells are derived from luminal lactiferous ductal epithelium (A) of breast tissue with retrograde extension of cancerous Paget cells into epidermis of overlying nipple (B). Enlarged circle shows details that reveal thickening both of lining epithelium of breast duct and of nipple skin.
Biopsy-proven Paget disease involving nipple of 56-year-old woman. Patient noted erythematous, swollen, enlarged nipple with focal ulceration and oozing; occasional serosanguineous discharge and bleeding were present. Patient was later found to have palpable breast mass and mammography results positive for subareolar microcalcification; no auxiliary lymphadenopathy was found. Patient was treated by simple mastectomy. At 5-year follow-up, she was alive, without recurrent or metastatic tumor.
Mammary Paget disease (PD) affecting 48-year-old woman. Patient had experienced prolonged history of chronic eczematous dermatitis of nipple and areolar area for several years. Lesion did not respond to topical treatment, and it progressively distorted nipple with expansion into surrounding skin. Note markedly scaly, crusted, and deformed nipple with thickened, irregularly outlined adjacent nipple-areola complex. Excisional biopsy confirmed diagnosis of mammary PD. Patient developed infiltrating ductal carcinoma of underlying breast tissue with axillary lymph metastasis. She was treated by mastectomy and radiation. No metastatic tumor was noted in axillary lymph node. Patient was alive and well 3 years after treatment.
Nipple invagination, deformed nipple-areola complex, marked erythema, and alternating hyperpigmentation and hypopigmentation noted in adjacent skin of breast in 65-year-old woman with biopsy-proven Paget disease. Note focal scaling of previous biopsy site. Nipple changes were associated with intraductal carcinoma of breast. Patient was treated by conservative excision of lesion and lumpectomy for in situ carcinoma. No recurrence or metastatic disease was noted at 6-year follow-up.
Photomicrograph of mammary Paget disease lesion. Note nests of malignant Paget cells predominantly involving lower layers of epidermis. Cytoplasm of tumor cells contains abundant pale-staining, granular, mucinous material. Occasional small glandular structures can be seen within malignant cell nests (hematoxylin-eosin, ×100).
Low-power view of transmission electron micrograph displaying malignant Paget cells in lower layer of epidermis. Note large Paget cell containing ovoid nucleus (N), scanty nuclear chromatin, large nucleolus, and abundant pale-staining cytoplasm with smooth and rough endoplasmic reticulum (arrow), scattered enlarged mitochondria, free ribosomes, and lysosomes. No desmosomal attachments are seen between Paget cells and adjacent keratinocytes. Tonofilaments are seen in keratinocytes (uranyl acetate and lead citrate, ×5,500).
Photomicrograph of intraductal carcinoma of breast underneath Paget disease of nipple in 56-year-old woman. Note expansion of ductal lumen, which is filled with irregularly sized tumor cells of ductal epithelial origin. Nuclear hyperchromatism and gland-in-gland (cribriform) pattern are evident. Tumor was detected by positive mammogram result depicting focus of microcalcification beneath nipple.
Photomicrograph of malignant melanoma in situ of skin displays prominent intraepidermal pagetoid spread. Note that melanoma cells are present in all layers of epidermis, mostly in single units. Cytoplasm of melanoma cells is vacuolated. Moderate upper dermal chronic inflammatory infiltrate is present (hematoxylin-eosin, original magnification ×250). S-100 protein and homatropine methylbromide immunostains are positive in melanoma cells, whereas carcinoembryonic antigen is negative. No epithelial mucin is seen in these tumor cells.
Schematic diagram of female breast depicting widely accepted concept of pathogenesis of mammary Paget disease. Malignant Paget cells are derived from luminal lactiferous ductal epithelium (A) of breast tissue with retrograde extension of cancerous Paget cells into epidermis of overlying nipple (B). Enlarged circle shows details that reveal thickening both of lining epithelium of breast duct and of nipple skin.
Biopsy-proven Paget disease involving nipple of 56-year-old woman. Patient noted erythematous, swollen, enlarged nipple with focal ulceration and oozing; occasional serosanguineous discharge and bleeding were present. Patient was later found to have palpable breast mass and mammography results positive for subareolar microcalcification; no auxiliary lymphadenopathy was found. Patient was treated by simple mastectomy. At 5-year follow-up, she was alive, without recurrent or metastatic tumor.
Mammary Paget disease (PD) affecting 48-year-old woman. Patient had experienced prolonged history of chronic eczematous dermatitis of nipple and areolar area for several years. Lesion did not respond to topical treatment, and it progressively distorted nipple with expansion into surrounding skin. Note markedly scaly, crusted, and deformed nipple with thickened, irregularly outlined adjacent nipple-areola complex. Excisional biopsy confirmed diagnosis of mammary PD. Patient developed infiltrating ductal carcinoma of underlying breast tissue with axillary lymph metastasis. She was treated by mastectomy and radiation. No metastatic tumor was noted in axillary lymph node. Patient was alive and well 3 years after treatment.
Nipple invagination, deformed nipple-areola complex, marked erythema, and alternating hyperpigmentation and hypopigmentation noted in adjacent skin of breast in 65-year-old woman with biopsy-proven Paget disease. Note focal scaling of previous biopsy site. Nipple changes were associated with intraductal carcinoma of breast. Patient was treated by conservative excision of lesion and lumpectomy for in situ carcinoma. No recurrence or metastatic disease was noted at 6-year follow-up.
Photomicrograph of malignant melanoma in situ of skin displays prominent intraepidermal pagetoid spread. Note that melanoma cells are present in all layers of epidermis, mostly in single units. Cytoplasm of melanoma cells is vacuolated. Moderate upper dermal chronic inflammatory infiltrate is present (hematoxylin-eosin, original magnification ×250). S-100 protein and homatropine methylbromide immunostains are positive in melanoma cells, whereas carcinoembryonic antigen is negative. No epithelial mucin is seen in these tumor cells.
Photomicrograph of mammary Paget disease lesion. Note nests of malignant Paget cells predominantly involving lower layers of epidermis. Cytoplasm of tumor cells contains abundant pale-staining, granular, mucinous material. Occasional small glandular structures can be seen within malignant cell nests (hematoxylin-eosin, ×100).
Low-power view of transmission electron micrograph displaying malignant Paget cells in lower layer of epidermis. Note large Paget cell containing ovoid nucleus (N), scanty nuclear chromatin, large nucleolus, and abundant pale-staining cytoplasm with smooth and rough endoplasmic reticulum (arrow), scattered enlarged mitochondria, free ribosomes, and lysosomes. No desmosomal attachments are seen between Paget cells and adjacent keratinocytes. Tonofilaments are seen in keratinocytes (uranyl acetate and lead citrate, ×5,500).
Photomicrograph of intraductal carcinoma of breast underneath Paget disease of nipple in 56-year-old woman. Note expansion of ductal lumen, which is filled with irregularly sized tumor cells of ductal epithelial origin. Nuclear hyperchromatism and gland-in-gland (cribriform) pattern are evident. Tumor was detected by positive mammogram result depicting focus of microcalcification beneath nipple.