Follicle-Stimulating Hormone Abnormalities

Practice Essentials

Follicle-stimulating hormone (FSH) is a glycoprotein gonadotropin secreted by the anterior pituitary in response to gonadotropin-releasing hormone (GnRH) released by the hypothalamus. The pituitary gland also secretes luteinizing hormone (LH), another gonadotropin. FSH and LH are composed of alpha and beta subunits. The specific beta subunit confers the unique biologic activity. FSH and LH bind to receptors in the testis and ovary and regulate gonadal function by promoting sex steroid production and gametogenesis.^[1]

In men, LH stimulates testosterone production from the interstitial cells of the testes (Leydig cells). FSH stimulates testicular growth and enhances the production of an androgen-binding protein by the Sertoli cells, which are a component of the testicular tubule necessary for sustaining the maturing sperm cell. This androgen-binding protein causes high local concentrations of testosterone near the sperm, an essential factor in the development of normal spermatogenesis. Sertoli cells, under the influence of androgens, also secrete inhibin, a polypeptide, which may help to locally regulate spermatogenesis. Hence, maturation of spermatozoa requires FSH and LH.

In women, LH stimulates estrogen and progesterone production from the ovary. A surge of LH in the midmenstrual cycle is responsible for ovulation, and continued LH secretion subsequently stimulates the corpus luteum to produce progesterone. Development of the ovarian follicle is largely under FSH control, and the secretion of estrogen from this follicle is dependent on FSH and LH. The granulosa cells of the ovary secrete inhibin, which plays a role in cellular differentiation.

FSH and LH secretion are affected by a negative feedback from sex steroids. Inhibin also has a negative feedback on FSH selectively. High-dose testosterone or estrogen therapy suppresses FSH and LH. Primary gonadal failure in men and women leads to high levels of FSH and LH, except in selective destruction of testicular tubules with subsequent elevation of only FSH, as in Sertoli-cell-only syndrome. Similarly, any process leading to a low FSH level also simultaneously results in a low LH level, except in rare instances of isolated FSH deficiency or isolated LH deficiency in fertile eunuch syndrome.

Diagnosis

Perform additional laboratory studies in men presenting with low follicle-stimulating hormone (FSH) levels, including the following:

• LH and testosterone levels
• Prolactin levels
• Serum estradiol levels: This is performed to exclude an estrogen-secreting tumor (testes, adrenals) in men with low FSH levels and feminizing features, including gynecomastia
• Semen analysis: This is performed to assess fertility

In men presenting with high FSH levels, the underlying etiology is related to primary hypogonadism or a gonadotroph adenoma. Therefore, the following lab tests are indicated:

• LH and testosterone levels
• In patients with gonadotroph adenomas, other pituitary hormone levels must also be assessed because macroadenomas may induce hypopituitarism; serum TSH and free thyroxine (T4), morning cortisol and adrenocorticotropic hormone (ACTH), prolactin, and, occasionally, dynamic testing for growth hormone (GH) may be necessary
• Peripheral leukocyte karyotype: This is obtained in men with congenital primary hypogonadism to determine if Klinefelter syndrome is present

In women presenting with low FSH levels, additional testing should include determination of LH, estradiol, and prolactin levels. Thyroid disease should be excluded by measuring TSH and free T4. If hirsutism is present, serum testosterone and dehydroepiandrosterone sulfate (DHEAS) testing should be performed. Moreover, additional testing such as determination of the serum 17-hydroxyprogesterone level before and after ACTH stimulation may be performed if congenital adrenal hyperplasia is suggested.

In women with high FSH levels, the differential diagnosis is either ovarian failure or gonadotroph adenoma. The following points should be remembered:

• In women with ovarian failure, both FSH and LH levels rise; in women with gonadotroph adenomas, FSH levels are usually high, but LH levels remain within reference ranges; other pituitary hormone abnormalities may be present
• If the diagnosis of ovarian failure is confirmed in patients younger than 30 years, a karyotype evaluation should be performed to exclude Turner syndrome or the presence of Y chromatin material because of the high risk of gonadal tumors, mandating gonadectomy
• In the presence of a normal karyotype, autoimmune disease is likely (30% of these patients); therefore, assessment for autoimmune disorders, including thyroid or adrenal disease, is important; testing may include TSH, antithyroid antibodies, morning serum cortisol, and ACTH evaluations, followed by an ACTH stimulation test if necessary

In men or women with low FSH, high prolactin, or high FSH levels (the latter being suggestive of gonadotroph adenoma in the appropriate clinical setting), a magnetic resonance imaging (MRI) scan of the pituitary gland must be obtained.

In women with clinical features and laboratory findings suggestive of an ovarian tumor but with negative results from imaging studies, laparoscopy may be performed to help locate ovarian masses.

Management

Medical treatment in patients with abnormal levels of FSH depends on the underlying etiology. In women with primary (ovarian) or secondary (pituitary) hypogonadism, hormone replacement therapy may be administered (estrogen and progesterone). However, a study by the Women's Health Initiative showed health risks from using estrogens plus progesterone.^[2]

In men with primary (testes) or secondary (pituitary) hypogonadism, testosterone replacement therapy is administered, either intramuscularly or with patches or gel.

Surgery is the treatment of choice for patients with gonadotroph adenomas, adrenal tumors, or gonadal tumors, unless contraindicated for other medical reasons.

• References

Pathophysiology

Follicle-stimulating hormone (FSH) abnormalities are divided into 2 major groups (low and high), depending on FSH levels.

Causes of low FSH level (hypogonadotropic hypogonadism or secondary hypogonadism)

See the list below:

• Congenital: Sexual differentiation is normal. In men, phallic development may be subnormal, resulting in a micropenis. Pubertal development is diminished or even absent, depending on the degree of gonadotropin deficiency.

  • Isolated idiopathic hypogonadotropic hypogonadism: This usually results from GnRH deficiency, with absence of any other abnormalities. Mutations are shown in the image below. FSH and LH levels are low.^[3]

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    Human G protein-coupled receptor 54 (GPR54) receptor model. Mutations identified in patients with idiopathic hypogonadotropic hypogonadism are indicat....

  • Kallmann syndrome: This is characterized by hypogonadotropic hypogonadism and 1 or more nongonadal congenital abnormalities, including anosmia, red-green blindness, midline facial abnormalities (eg, cleft palate), urogenital tract abnormalities, and neurosensory hearing loss. Hypogonadism in this syndrome is a result of deficient hypothalamic secretion of GnRH. Most cases are sporadic, but familial cases also occur. It is caused by mutations in the KAL gene.^[4] Other mutations have been described, such as FGFR1 (KAL2), GPRS54, PROK-2, PROKR-2, CHD-7, and FGF-8.^[5] See the image below.

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    This is a frequently sampled serum luteinizing hormone (LH) profile in a male patient with Kallmann syndrome (KS), compared with that in a healthy ind....

  • Idiopathic hypogonadotropic hypogonadism associated with mental retardation: Several syndromes (eg, Prader-Willi syndrome) have been described in which hypogonadotropic hypogonadism is associated with retardation and other abnormalities, including obesity.
  • Craniopharyngiomas: These tumors arise from remnants of the Rathke pouch, which is the diverticulum of the roof of the embryonic oral cavity that normally gives rise to the anterior pituitary. Craniopharyngiomas are congenital malformations present at birth and gradually grow over the years. Approximately 75% arise in the suprasellar region. The most common presentation is due to increased intracranial pressure, including headaches and visual-field defects. A study by Zhang et al using the Surveillance, Epidemiology, and End Results (SEER) database found bimodal incidence peaks for craniopharyngiomas, with one peak for ages 20 years and younger and another for ages 40-65 years.^[6]
  • Combined pituitary hormone deficiency: This results from a rare mutation in the gene encoding a transcription factor (PROP1), which is necessary for the differentiation of a cell type that is a precursor to somatotroph, lactotroph, thyrotroph, and gonadotroph cells, thus resulting in deficiencies in prolactin, thyroid-stimulating hormone (TSH), growth hormone (GH), FSH, and LH. A study by Otto et al found that of 83 patients with childhood-onset isolated GH deficiency, 37 (44.6%) developed combined pituitary hormone deficiency over a median 5.4-year follow-up, with FSH and LH deficiencies having a prevalence of 38% in these patients.^[7]
  • Fertile eunuch syndrome: This is thought to represent an incomplete form of GnRH deficiency in men, in which an isolated and partial LH deficiency is present with low testosterone and normal FSH levels, resulting in preservation of spermatogenesis.
  • Abnormal beta subunit of LH: This is a rare mutation in the LH beta subunit gene.
  • Abnormal beta subunit of FSH: This is a rare mutation in the gene for the beta subunit of FSH, resulting in a low FSH level. This condition is encountered only in women but has been studied in male mice in which the FSH beta subunit gene has been knocked out. These mice have oligospermia but are fertile.^[8]

   

  

   
• Acquired: This can be caused by any disease that affects the hypothalamic-pituitary axis, impairing the secretion of GnRH, FSH, or LH.

  • Mass lesions: These include pituitary adenomas (as shown below), cysts, and metastatic cancer to the sella (breast in women, lung and prostate in men). These masses may cause temporary or permanent damage by extrinsic compression of pituitary cells. Hypothalamic tumors may lead to delayed puberty, hypogonadism, and obesity, originally called Fröhlich syndrome or adiposogenital dystrophy. The presence of obesity indicates that the appetite-regulating regions of the hypothalamic have been damaged.

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    Magnetic resonance imaging (MRI) scan of pituitary macroadenoma.

  • Hypothalamic/pituitary surgery: If sufficient normal tissue is excised inadvertently, symptomatic hypogonadism may ensue initially, followed by dysfunction of other pituitary cells.
  • Hypothalamic/pituitary radiation: This may lead to multiple hormonal deficiencies, including FSH and LH.
  • Infiltrative lesions: Hemochromatosis, sarcoidosis, histiocytosis, and lymphoma can cause hypogonadism by involving the hypothalamic/pituitary region.
  • Lymphocytic hypophysitis: This is characterized by lymphocytic infiltration and destruction of the pituitary cells. Thought to be autoimmune in nature, lymphocytic hypophysitis is an uncommon disorder that usually occurs in women, often in the postpartum period. However, cases have also been described in men.
  • Infections: Meningitis, especially tuberculous, is a rare cause of hypogonadism in the United States.
  • Pituitary apoplexy: This is a sudden and severe hemorrhage into the pituitary, which can result in varying degrees of hypopituitarism, excruciating headaches, visual changes, and altered mental status.
  • Trauma: Head trauma of sufficient severity to fracture the skull base can sever the hypothalamic-pituitary stalk, preventing GnRH from reaching the pituitary, thus decreasing FSH and LH release.
  • Glucocorticoid excess: Exogenous or endogenous (Cushing syndrome) glucocorticoid excess can lead to hypogonadotropic hypogonadism. Direct inhibition of testosterone secretion may also occur at the testicular level.
  • Hyperprolactinemia: This can result from a pituitary adenoma, renal or liver insufficiency, primary hypothyroidism, or some drugs (eg, neuroleptics). Hyperprolactinemia can suppress GnRH secretion through a central dopamine-related mechanism. In addition to hypogonadism, this condition can also manifest as galactorrhea and as gynecomastia in men.
  • Primary hypothyroidism: This can lead to hypogonadism through hyperprolactinemia. A low thyroxine (T4) level results in a high thyrotropin-releasing hormone (TRH) level, which stimulates prolactin secretion.
  • Critical illness: Surgery, myocardial infarction, or other illness can cause transient hypogonadotropic hypogonadism, with resolution upon recovery.
  • Excessive exercise: This can cause a functional hypothalamic hypogonadism in men, analogous to women with functional hypothalamic amenorrhea.
  • Sex steroid–secreting tumors: These may be adrenal, testicular, or ovarian in origin, or, they may result from adrenal rest tumors. The excessive amount of testosterone or estradiol can inhibit FSH and LH secretion.
  • Intentional (iatrogenic) secondary hypogonadism: Prolonged administration of high doses of anabolic steroids (by athletes) or GnRH analogs (for prostate cancer) can cause low FSH or LH levels. Recovery may take many months or years after cessation of the drug. Also, women who discontinue oral contraceptives may have post-pill amenorrhea; recovery of the gonadotropin axis may take up to a year.
  • Empty sella: This term refers to an enlarged sella turcica that is not entirely filled with pituitary tissue, either from a defect in the diaphragm sella (allowing cerebrospinal fluid pressure to enlarge the sella) or secondary to a mass that is removed by surgery, radiation, or infarction.
  • Pituitary infarction: This condition rarely occurs in males; but, when present, it primarily manifests in older patients with vascular insufficiency during coronary artery bypass surgery. In women, it can occur postpartum as Sheehan syndrome, usually after substantial blood loss during childbirth. This condition manifests as partial or complete hypopituitarism, depending on the hormonal deficiencies; a low FSH or LH level causing amenorrhea is the most frequent cause.
  • Chronic systemic diseases: Cirrhosis, chronic renal failure, and AIDS may lead to hypogonadism, which has a dual mechanism, ie, primary and secondary.
  • Anorexia nervosa: In women, significant weight loss, up to 10% below the ideal body weight, may lead to functional hypothalamic amenorrhea.
  • Congenital adrenal hyperplasia: This is due to 21-hydroxylase deficiency or rarely to 11-hydroxylase deficiency; both lead to high levels of androgens, which can lower FSH and LH levels.
  • Acute alcohol ingestion: This may lead to primary or secondary hypogonadism.
  • Idiopathic: No cause is identified in some men and women with acquired secondary hypogonadism. The cause may be autoimmune in origin.
  • Type 2 diabetes: Research has indicated that low concentrations of testosterone, LH, and FSH are prevalent in patients with type 2 diabetes who are obese.^[9] Evidence suggests that inflammation may play an important part in this phenomenon.

   

  

   

Causes of high FSH level

Primary hypogonadism: This can be congenital or acquired

• Congenital: Sexual differentiation in men may vary from pseudohermaphrodism to a male with only a micropenis and lack of full pubertal development. In women, sexual differentiation is normal but puberty is delayed or absent.

  • Klinefelter syndrome: This syndrome is the most common congenital abnormality causing primary hypogonadism in men. The typical genotype is 47,XXY. The clinical presentation includes infertility, small and firm testes, and low testosterone with high FSH and LH levels (see the image below). Males with Klinefelter syndrome usually present in their prepubertal years.^{[10, 11, 12]}

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    Adolescent male with Klinefelter syndrome who has female-type distribution of pubic hair, as well as testicular dysgenesis.

  • Other chromosomal abnormalities: These result in testicular hypofunction; they include the 46,XY/XO and the 47,XYY karyotypes.
  • Mutation in the FSH receptor gene in men and women: This mutation is rare and results in low sperm count with a high FSH level in men. Women present with amenorrhea and elevated FSH.^[13]
  • Cryptorchidism: This refers to undescended testes, as in the image below. The clinical consequences depend on whether 1 or both testes are cryptorchid. If only 1 testis is affected, the sperm count is subnormal in almost 30% of patients and the FSH level is slightly elevated. If both testes remain undescended, the sperm count is usually severely subnormal with a high FSH level and low serum testosterone. A study by Rohayem et al indicated that in males with undescended testes, a positive correlation exists between the age of correction and levels of FSH and LH.^[14]

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    Hypoplastic right hemiscrotum in a patient with an undescended right testis.

  • Disorders of androgen biosynthesis: This involves mutations of the genes that encode the enzymes necessary for testosterone biosynthesis. They result in incomplete virilization, low sperm count, low testosterone level, and high LH and FSH levels.
  • Sertoli-cell-only syndrome: The characteristic features are complete, or almost complete, absence of germ cells in all seminiferous tubules. Leydig cells are only mildly impaired. These men have azoospermia with high FSH levels. LH and testosterone levels are normal. The cause has not been identified, but it is thought to be a congenital absence or early neonatal loss of the germ cells.
  • End organ resistance to androgens: This is due to androgen receptor defects. In its complete form, it is called testicular feminization. Affected individuals are genetic males but phenotypic females. The testes are located in the labia, the inguinal canal, or the abdomen. Testosterone and LH levels are high. The FSH level is normal or slightly increased.
  • Turner syndrome: This is 45,X gonadal dysgenesis in women. It is characterized by short stature, sexual infantilism, and distinctive features. These women have primary amenorrhea and infertility. They have distinctive facies, including micrognathia, epicanthal folds, and prominent low-set ears. The neck is short and broad with webbing in 25-40%. They can have associated cardiac, renal, or skeletal abnormalities.^{[15, 16]}
  • Myotonic dystrophy: This is an autosomal dominant disorder that leads to muscle atrophy accompanied by hypogonadism manifesting as small testes and decreased sperm production. The FSH level is high in most of these patients; approximately half have low testosterone and high LH levels.

   

  

   
• Acquired

  • Infections: The most common is mumps orchitis. The seminiferous tubules are almost always severely affected, often resulting in infertility, especially with bilateral testicular involvement. The Leydig cells may also be damaged, resulting in decreased testosterone production with high LH levels.
  • Radiation: This mostly damages the seminiferous tubules or the ovaries. The degree of damage is proportionate to the level of radiation exposure.
  • Antineoplastic agents: As with cyclophosphamide, chlorambucil, cisplatin, and carboplatin may decrease the sperm count by destruction of the seminiferous tubules. Less commonly, testosterone secretion also declines. Recovery may occur over the long-term. Similarly, in women, chemotherapy may lead to ovarian failure.
  • Chemicals: Chemicals such as dibromodichloropropane can decrease spermatogenesis.
  • Glucocorticoids: These can lead to hypogonadism via inhibition of the pituitary and testes.
  • Ketoconazole: This is an antifungal drug that inhibits testosterone biosynthesis.
  • Suramin: This is an antiparasitic drug that can block testosterone synthesis by the Leydig cells.
  • Trauma: Injuries can be sufficiently severe to damage both seminiferous tubules and Leydig cells.
  • Testicular torsion: Torsion of more than 8 hours duration may lead to a low sperm count. Even if the torsion involves only 1 testis, both testes may be damaged; the mechanism is not known.
  • Chronic systemic diseases: Cirrhosis, chronic renal failure, and AIDS may lead to hypogonadism, both primary and secondary. Bilateral aortofemoral anastomosis in men may lead to decreased blood supply to the testes, predominantly affecting the seminiferous tubules.
  • Ovarian failure: Failure occurs when the supply of oocytes and surrounding follicles is depleted. This usually happens at approximately age 50 years in American women during the course of normal menopause. Premature depletion of oocytes prior to age 40 years is called premature menopause or premature ovarian failure. The lack of ovarian function leads to absolute estrogen deficiency, endometrial atrophy, and the absence of menstruation. Loss of negative feedback of estradiol on the hypothalamus and pituitary and the loss of inhibin result in high FSH levels.
  • Autoimmune damage: This is due to antisperm antibodies. It may be part of an autoimmune polyglandular syndrome.
  • Idiopathic: Many men and women with primary hypogonadism have idiopathic disease, and the cause is never identified. The cause may be autoimmune in origin.
  • Gonadotroph adenomas: These are the most common pituitary macroadenomas. They usually do not cause a recognizable clinical endocrine syndrome. They manifest as visual impairment, headaches, and deficiency of pituitary hormones due to compression of nonadenomatous pituitary cells by the macroadenoma. The gonadotroph adenoma itself can oversecrete FSH, LH, or one of the subunits (alpha or beta).

   

  

   

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Epidemiology

Frequency

International

The frequency depends on each disease and its manifestation of high or low follicle-stimulating hormone levels. Refer to the appropriate articles for prevalence rates.

Mortality/Morbidity

The resulting morbidity and mortality are usually related to the conditions that cause the alterations in follicle-stimulating hormone secretion.

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History

The symptomatology depends on the disease causing the low or high follicle-stimulating hormone (FSH) levels and varies between men and women.

In men presenting with low FSH levels leading to secondary hypogonadism or high FSH levels resulting from primary hypogonadism, the history reveals erectile dysfunction, decreased libido, infertility, and low energy.

• In those with hyperprolactinemia, galactorrhea and/or gynecomastia may be present.
• Depending on the timing of the FSH abnormality, further questioning may indicate ambiguous genitalia at birth or a failure to undergo or complete puberty.
• Anosmia suggests Kallmann syndrome.
• Visual abnormalities, headaches, and other hormonal deficiencies suggest a mass or a destructive process involving the pituitary.

In men presenting with high FSH levels due to a gonadotroph adenoma, symptoms result from the mass effect (eg, headaches, visual impairment, hormonal deficiencies). However, erectile dysfunction and infertility may occur secondary to low LH levels caused by compression of the normal gonadotroph cells.

In women presenting with low FSH levels or high FSH levels secondary to ovarian failure, manifestations include oligomenorrhea or amenorrhea.

• Galactorrhea may be present in the setting of high prolactin levels.
• Symptoms of other pituitary hormone deficiencies may also be evident if a mass or a destructive process involves the pituitary gland.
• Women may have primary or secondary infertility.
• In women with ovarian failure, other symptoms may include hot flashes, sleep disturbance, mood swings, depression, vaginal dryness and dyspareunia, urinary incontinence, and urinary tract infections.

In women with high FSH levels from a gonadotroph adenoma, symptoms are frequently due to mass effect (eg, headaches, visual changes, hypopituitarism). However, a high FSH level may also lead to ovarian hyperstimulation in premenopausal women, with multiple ovarian cysts^[17] and a thickened endometrium; this leads to disturbed menstrual cycles, ie, oligomenorrhea or amenorrhea.

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Physical

The physical examination findings also depend on the disease that underlies the abnormal follicle-stimulating hormone level. The following important points must be remembered.

In men presenting with hypogonadism, findings depend on the timing of onset during life and on the duration of testosterone deficiency.

• In utero (if testosterone deficiency occurs in the first trimester): Male sexual differentiation is incomplete, resulting in pseudohermaphroditism. Complete lack of testosterone results in female external genitalia. Incomplete testosterone deficiency causes partial virilization with ambiguous genitalia.
• In utero (if testosterone deficiency occurs after the first trimester): Normal male sexual differentiation occurs but with micropenis/cryptorchidism at birth.
• Before puberty: Testosterone deficiency results in delayed and incomplete puberty.

  • Small testes (< 2.5 cm [normal = 4-7 cm])
  • Short phallus
  • High-pitched voice
  • Decreased muscle mass
  • Decreased body hair
  • Delayed bone age
  • Eunuchoid skeletal proportions: Eunuchoidism is a lack of testosterone during puberty that causes a delay in epiphyseal closure so that the continued presence of GH results in increased length of the long bones (ie, arm span greater than height, lower body segment [heel to pubis] longer than upper body segment [pubis to crown]).

   

  

   
• After puberty

  • Normal skeletal proportions and penile length
  • Soft testes
  • Decreased strength and muscle mass
  • Decreased rate of hair growth (facial, pubic, axillary)
  • Gynecomastia

   

  

   

In women presenting with hypogonadism (oligomenorrhea or amenorrhea), physical examination findings may include hirsutism and/or masculinization (eg, frontal balding, clitoromegaly, increased muscle mass) only if an excess of androgens is present, which is not frequent. The examination may reveal stigmata of Turner syndrome (eg, short stature, webbed neck, shield chest, wide carrying angle of the arms), short fourth metacarpals, cardiovascular anomalies (coarctation of aorta, bicuspid aortic valve), or evidence for other endocrine deficiencies or autoimmune diseases (eg, Addison disease, vitiligo, Hashimoto thyroiditis).

In men and women with pituitary macroadenomas, visual-field examination findings may be abnormal. Other signs of hypopituitarism may also be detected upon examination.

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Causes

See Pathophysiology.

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Laboratory Studies

Perform additional laboratory studies in men presenting with low follicle-stimulating hormone (FSH) levels.

• Regarding LH and testosterone, most patients with low FSH levels also have low LH and low testosterone levels. A few cases of isolated FSH deficiency exist in which LH and testosterone levels are within reference ranges but the sperm count is low.
• For prolactin, patients with high prolactin levels frequently manifest low FSH, LH, and testosterone levels, all of which may resolve with normalization of prolactin levels.
• In men with low FSH levels and feminizing features, including gynecomastia, serum estradiol measurement is indicated to exclude an estrogen-secreting tumor (testes, adrenals).
• Semen analysis is performed to assess fertility.

In men presenting with high FSH levels, the underlying etiology is related to primary hypogonadism or a gonadotroph adenoma. Therefore, the following lab tests are indicated:

• For LH and testosterone, patients with primary hypogonadism have low testosterone levels with high LH and FSH levels. Patients with gonadotroph adenomas usually have high FSH levels with normal LH and testosterone levels; occasionally, LH levels can be high, but testosterone levels are also high. Gonadotroph adenomas that secrete FSH may induce compression of normal pituitary cells, leading to low LH and testosterone levels.
• In patients with gonadotroph adenomas, other pituitary hormone levels must also be assessed because macroadenomas may induce hypopituitarism. Serum TSH and free T4, morning cortisol and adrenocorticotropic hormone (ACTH), prolactin, and, occasionally, dynamic testing for GH may be necessary.
• Obtain a peripheral leukocyte karyotype in men with congenital primary hypogonadism to determine if Klinefelter syndrome is present.

In women presenting with low FSH levels, additional testing should include determination of LH, estradiol, and prolactin levels. Thyroid disease should be excluded by measuring TSH and free T4. If hirsutism is present, serum testosterone and dehydroepiandrosterone sulfate (DHEAS) testing should be performed. Moreover, additional testing such as determination of the serum 17-hydroxyprogesterone level before and after ACTH stimulation may be performed if congenital adrenal hyperplasia is suggested.

In women with high FSH levels, the differential diagnosis is either ovarian failure or gonadotroph adenoma. The following points should be remembered:

• In women with ovarian failure, both FSH and LH levels rise. In women with gonadotroph adenomas, FSH levels are usually high, but LH levels remain within reference ranges. Other pituitary hormone abnormalities may be present.
• If the diagnosis of ovarian failure is confirmed in patients younger than 30 years, a karyotype evaluation should be performed to exclude Turner syndrome or the presence of Y chromatin material because of the high risk of gonadal tumors, mandating gonadectomy.
• In the presence of a normal karyotype, autoimmune disease is likely (30% of these patients); therefore, assessment for autoimmune disorders, including thyroid or adrenal disease, is important. Testing may include TSH, antithyroid antibodies, morning serum cortisol, and ACTH evaluations, followed by an ACTH stimulation test if necessary.

• References

Imaging Studies

In men or women with low follicle-stimulating hormone (FSH), high prolactin, or high FSH levels (the latter being suggestive of gonadotroph adenoma in the appropriate clinical setting), an MRI scan of the pituitary gland must be obtained.

In women with very high DHEAS levels (>700 mcg/dL), perform CT scanning of the adrenals to exclude an androgen-secreting tumor.

In women with very high testosterone levels (>200 ng/mL), perform imaging studies of the ovaries (CT scan, ultrasound).

In men with high estradiol levels, imaging of the testes (ultrasound) should be performed and, subsequently, the adrenals (CT scan) if the testicular ultrasound findings are normal.

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Procedures

In women with clinical features and laboratory findings suggestive of an ovarian tumor but with negative results from imaging studies, laparoscopy may be performed to help locate ovarian masses, which could be small and are frequently difficult to detect using routine imaging.

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Medical Care

Medical treatment in patients with abnormal levels of follicle-stimulating hormone (FSH) depends on the underlying etiology. Appropriate articles are available that contain detailed discussions. However, the following points deserve mention:

In women with primary (ovarian) or secondary (pituitary) hypogonadism, hormone replacement therapy may be administered (estrogen and progesterone). However, a study by the Women's Health Initiative showed health risks from using estrogens plus progesterone in 16,608 postmenopausal women over 5.2 years of follow-up. Excess risks attributable to estrogens plus progesterone included more cardiovascular events, strokes, pulmonary emboli, and invasive breast cancers, but less risk of colorectal cancer. Hormone replacement therapy should take into account all of these factors. Also, other pituitary deficiencies (eg, thyroid, adrenal) must be corrected in the appropriate setting.^[2]

In men with primary (testes) or secondary (pituitary) hypogonadism, testosterone replacement therapy is administered, either intramuscularly or with patches or gel. Again, other pituitary deficiencies must be corrected.

In men and women, treatment targeted at the underlying etiology may suffice. In patients with prolactinomas, medical treatment with a dopamine agonist lowers prolactin levels and may normalize FSH, LH, and testosterone/estradiol levels.^[18]  In patients with hemochromatosis, repeated phlebotomy to remove iron may reverse the gonadotropin deficiency.

A report on the use of recombinant human FSH (rhFSH) in the treatment of male infertility found that the total mobile sperm count (TMSC) rose in men with isolated FSH deficiency who received rhFSH therapy.^[19] The investigators studied 61 infertile men, including 13 patients with isolated FSH deficiency, who received 100-150 IU of rhFSH 2-3 times per week. The TMSC rose from 6.64 (±3.27) million to 32.4 (±9.09) million in the isolated FSH deficiency patients.

A study by Bry-Gauillard et al of women with isolated hypogonadotropic hypogonadism in association with Kallmann syndrome found that a significant rise in serum estradiol and inhibin B concentrations, as well as an increase in the number of larger antral follicles, occurred via ovarian stimulation with rhFSH. While a rise was also seen in the anti-Müllerian hormone level and smaller antral follicle count with rhFSH administration, these values subsequently fell even with continued stimulation.^[20]

A study by Zhang et al indicated that in patients with idiopathic hypogonadotropic hypogonadism, the use of sequential urinary FSH (uFSH)/human chorionic gonadotropin (hCG) injections is as effective as the administration of continual uFSH/hCG injections in inducing spermatogenesis and masculinization.^[21]

Hormone replacement therapy

Hormone replacement therapy must be monitored carefully. Women on estrogen therapy who have an intact uterus should receive progesterone therapy to prevent endometrial hyperplasia, and they should have yearly breast examinations and mammograms as indicated.

Patients should have yearly Papanicolaou tests (Pap smears).

If vaginal bleeding occurs while on therapy, an endometrial biopsy and/or dilation and curettage should be considered to determine the presence of endometrial cancer.

Men on testosterone replacement therapy should have yearly prostate-specific antigen tests and regular rectal examinations to monitor for prostate carcinoma.

A study by Rohayem et al indicated that in adult males, the efficacy of gonadotropin replacement for hypogonadotropic hypogonadism is predicted by the cause of the condition. The study found, for example, that men with Kallmann syndrome had the worst responses, while responses in males with congenital/infancy-acquired multiple pituitary hormone deficiency was also poor. However, men with postpubertally acquired multiple pituitary hormone deficiency had among the best outcomes.^[22]

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Surgical Care

Surgery is the treatment of choice for patients with gonadotroph adenomas, adrenal tumors, or gonadal tumors, unless contraindicated for other medical reasons.

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Consultations

Consultation with an endocrinologist or gynecologist is frequently helpful for appropriate diagnostic evaluation and management.

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Medication Summary

The goals of pharmacotherapy are to correct hormonal imbalances, to prevent complications, and to reduce morbidity.

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Conjugated estrogens (Premarin)

• Dosing, Interactions, etc.

Clinical Context:  Restore estrogen levels to concentrations that induce negative feedback at gonadotrophic regulatory centers, which, in turn, reduces FSH release from the pituitary gland. Vaginal estrogen creams may alleviate vaginal dryness and urogenital symptoms, but systemic absorption is not usually sufficient to provide systemic effect.

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Estradiol (Climara Transdermal, Estrace, Estraderm Transdermal, Gynodiol)

• Dosing, Interactions, etc.

Clinical Context:  Restores estrogen levels to concentrations that induce negative feedback at gonadotrophic regulatory centers, which, in turn, reduces FSH release from pituitary. Vaginal estrogen creams may alleviate vaginal dryness and urogenital symptoms, but systemic absorption is not usually sufficient to provide systemic effect.

• References

Medroxyprogesterone (Cycrin, Provera)

• Dosing, Interactions, etc.

Clinical Context:  Progestins stop endometrial cell proliferation, allowing organized sloughing of cells after withdrawal; typically does not stop acute bleeding episode but produces a normal bleeding episode following withdrawal.

• References

Testosterone (Delatestryl injection, AndroGel, Androderm, Testoderm TTS)

• Dosing, Interactions, etc.

Clinical Context:  Promotes the growth and development of the male sex organs and maintains secondary sex characteristics in androgen-deficient males

• References

Class Summary

Estrogen is used to induce negative feedback at gonadotrophic regulatory centers, which, in turn, reduces release of gonadotropins. Progestins are used to prevent endometrial hyperplasia in patients with an intact uterus. Testosterone is used in males to restore sexual function and to replace the benefits of endogenous testosterone.

• References